大师作文网英语翻译Here,we report on the optimisation and validation of a l
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英语翻译
Here,we report on the optimisation and validation of a liquid chromatographic method for the determination of 12 biologically active amines from
vegetal food products in a single 40-min run.The suitability of the method was checked in five vegetal products of distinct matrix:spinach (leaves),
hazelnut (high protein and fat content),banana,potato (high starch content),and milk chocolate (processed).Sample preparation consisted of a
0.6M perchloric acid extraction from a minced homogeneous aliquot.For samples with high starch content,a previous mild hydrolytic treatment
was required to prevent gel formation.The range of linearity was from 0.1 to 10 mg/l,except for serotonin and spermine (from 0.5 to 10 mg/l),
and the correlation coefficient was higher than 0.997 (P < 0.001) for all standard curves.The detection limits and the determination limit were
below 0.07 and 0.2 mg/l,respectively,except for spermine,which was 0.14 and 0.4 mg/l.The precision of the method was satisfactory; the relative
standard deviation obtained for each amine in each product was acceptable according to Horwitz.Recovery was between 77 and 110% for all
amines,irrespective of the product.
Here,we report on the optimisation and validation of a liquid chromatographic method for the determination of 12 biologically active amines from
vegetal food products in a single 40-min run.The suitability of the method was checked in five vegetal products of distinct matrix:spinach (leaves),
hazelnut (high protein and fat content),banana,potato (high starch content),and milk chocolate (processed).Sample preparation consisted of a
0.6M perchloric acid extraction from a minced homogeneous aliquot.For samples with high starch content,a previous mild hydrolytic treatment
was required to prevent gel formation.The range of linearity was from 0.1 to 10 mg/l,except for serotonin and spermine (from 0.5 to 10 mg/l),
and the correlation coefficient was higher than 0.997 (P < 0.001) for all standard curves.The detection limits and the determination limit were
below 0.07 and 0.2 mg/l,respectively,except for spermine,which was 0.14 and 0.4 mg/l.The precision of the method was satisfactory; the relative
standard deviation obtained for each amine in each product was acceptable according to Horwitz.Recovery was between 77 and 110% for all
amines,irrespective of the product.
在此,我们报告验证最佳化和液相色谱法测定生物活性胺12 植物食品在一个40-min跑.该方法的适用性五植物产品了不同的矩阵:菠菜(离开),榛子(高蛋白质和脂肪含量)、香蕉、土豆(高淀粉含量),牛奶巧克力(加工).由一个样品制备 酸消解提取0.6均匀aliquot碎了.为样本,与高淀粉含量、先前的温和的水解治疗 必须防止凝胶的形成.是线性的范围从0.1至10毫克/升,除了5 -羟色胺和spermine(在0.5 10毫克/升),及相关系数是高于(P < 0.001)0.997所有标准曲线.检测范围和决心限制 低于0.07 0.2,分别毫克/升,除了spermine 0.14毫克/升)和0.4.分析方法的精密度是令人满意的,相对的 标准偏差为每个胺得到在每一个产品都是可以接受的根据Horwitz.恢复77与110%之间 胺、不分产品.
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