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如何配置km8p培养基?

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如何配置km8p培养基?
KM8P培养基中的有机酸和维生素的母液怎么配置?因为他们有好多不能高温高压灭菌,有些不能溶于酸碱。
如何配置km8p培养基?
不知下面这个是否有用
Kao K N,Michayluk M R.Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media.planta,1975.126:105-110.
配方:
KAO & Michayluk with vitami
Ammonium Nitrate 600.0 mg/L
Boric Acid 3.0 mg/L
Calcium Chloride,Anhydrous 453.0 mg/L
Cobalt Chloride 6H2O 0.025 mg/L
Cupric Sulfate 7H2O 0.025 mg/L
Na2 EDTA 37.25 mg/L
Ferrous Sulfate 7H2O 27.85 mg/L
Magnesium Sulfate 146.55 mg/L
Manganese Sulfate H2O 10.0 mg/L
Molybdic Acid,Sodium Salt H2O 0.25 mg/L
Potassium Chloride 300.0 mg/L
Potassium Iodide 0.75 mg/L
Potassium Nitrate 1900.0 mg/L
Potassium Phosphate,Monobasic 170.0 mg/L
Zinc Sulfate 7H2O 2.0 mg/L
p-Aminobenzoic Acid 0.02 mg/L
L-Ascorbic 2.0 mg/L
Biotin 0.01 mg/L
D-Calcium Pantothenate 1.0 mg/L
Choline Chloride 1.0 mg/L
Folic Acid 0.4 mg/L
myo-Inositol 100 mg/L
Nicotinamide 1.0 mg/L
Pyridoxine HCl 1.0mg/L
Riboflavin 0.2 mg/L
Thiamine HCl 1.0 mg/L
Vitamin A 0.01 mg/L
Vitamin B12 0.02 mg/L