英语翻译2.6.Expressible waterThe amount of expressible water (EW

英语翻译
2.6.Expressible water
The amount of expressible water (EW) for each treatment was measured.Samples of 3 g (±0.2 g) of fish gels were weighed and put between two layers of filter paper.Samples were placed at the bottom of 50 ml centrifuge tubes and centrifuged at 1000g for 15 min at 15 °C (Uresti,Ramı´rez,Lo´pez-Arias,& Va´zquez,2003).Immediately after centrifugation,the fish gel samples were weighed and the EW was calculated as:expressible water (%) = (Wi-Wf)/Wi*100,where Wi is the initial weight of gel,Wf is the final weight of gel.
2.7.TBA test
Method was based on Gomes,Silva,Nascimento,and Fukuma (2003).The TBA (2-thiobarbituric acid) solution was prepared by weighing 0.3 g of TBA and transferring in a 100 ml beaker with 90 ml distilled water.The beaker was placed in a water bath (80 °C) until complete dissolution.The solution was then quantitatively transferred to a 100 ml volumetric flask and the volume completed with distilled water so as to achieve a 0.021 M TBA solution.Minced sample (50 g) was blended after the addition of 6 ml of ethanolic solution of butylated hydroxytoluene (BHT,1 g/l) to prevent autoxidation.Aliquots of homogenized sample (10 g) were transferred to a flat-bottomed
flask and one drop of silicone anti-foaming agent added plus 2.5 ml of 4 N HCl and 97.5 ml of distilled water.This sample was then distilled and the first 50 ml of distillate collected.Distillation was carried out in triplicate.Then 5 ml of the distillate plus 0.6 ml of BHT (1 g/l) were added to 5 ml of 0.021 M TBA solution into a screw-cap test tube and heated in a water bath (90 °C) for 40 min for pink color development.The test tube was then cooled and the optical density was determined at 532 nm on a spectrophotometer (Spectrum-cn 722E,China) using control solution containing 5 ml distilled water,5 ml TBA solution and 0.6 ml BHT.TBA values were expressed as mg of malondialdehyde (MDA) per kg of sample.The concentration of
MDA was calculated from a standard curve using 1,1,3,3-tetraethoxy-propane (TEP) as the standard compound.

2.6.可表现的水
给每治疗的大量可表现水 (EW) 是标准的.3 g(± 0.2 g) 的样品 sh 凝胶是秤重和放在二之间层 lter 纸.样品被放置在那底部 50 毫升离心分离机管和离心分离机在 1000 g 15 分钟在 15 °C(Uresti,公羊?′ rez,瞧!′ pez-抒情调,& 伏-安培′ zquez,2003).在离心分离之后立刻,那 sh 凝胶样品是秤重和 EW 是有计划的当做:可表现的水 (%)=(Wi-Wf)/Wi*100,哪里 Wi 是那起始凝胶的重量,Wf 是那 nal 凝胶的重量.
2.7.TBA 测试
方法以 Gomes 、 Silva 、 Nascimento 和 Fukuma 为基础.(2003)TBA(2 thiobarbituric 的酸) 解决是准备被 TBA 的秤重 0.3 g 而且以被蒸馏的 90 毫升在一个 100 毫升大口杯中转移水.大口杯是放在一之内水沭浴 (80 °C) 直到完全的分解.解决然后数量地被转移到一 100 毫升测定体积的 为了要达成这 0.021 M TBA 解决,问和用蒸馏的水体积.切碎样品 (50 g) 被混合了在避免自然氧化的 butylated hydroxytoluene(BHT,1 g/l) 的 6 毫升 ethanolic 解决的附加之后.均质化的样品 (10 g) 的能整除是转移到一 在-根据
问和一下降矽树脂反起泡沫代理人附加的加号蒸馏的 2.5 毫升的 4 N HCl 和 97.5 毫升水.这个样品然后蒸馏和那 rst 50 毫升的 distillate 收集成的.蒸馏是被运的在一式三份.然后 5 毫升 distillate 加号 0.6 毫升的 BHT(1 g/l) 是附加的至 0.021 M TBA 解决的 5 毫升进入一之内螺丝钉-无边帽测试管和热的在一水沭浴 (90 °C) 为 40 分钟为粉红的彩色发展.测试管然后是冷却和光学的密度是坚决的在使用的一个分光光度计 (光谱-cn 的 722E,中国) 上的 532个 nm 控制包含 5 毫升的解决蒸馏水,5 毫升 TBA 解决和 0.6 毫升 BHT.TBA 价值是表达当做 malondialdehyde(MDA) 的毫克每一公斤样品.集中
MDA 是有计划的从标准曲线使用 1,1,3,3 tetraethoxy 的-丙烷 (TEP) 当做标准化合物.