大师作文网英语翻译The Sunfish amphiphiles were prepared from 4-picoline an
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英语翻译
The Sunfish amphiphiles were prepared from 4-picoline and the appropriate alkyl iodides in a three-step procedure according to the methods published previously.For the current study,an array of amphiphiles(1a-1f.Table1)was chosen,differing in both tail length and unsaturation of the tails.DOPE was purchased from Avanti Polar Lipids,Inc(Alabaster,AL).Nile Red was obtained from ACROS(Landameer,The Netherlands).Asample of 7.1-kilobase DNA was isolated feom Escherichia coli using the Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands).The plasmid concentration was determined spectrophotometrically by measuring the absorption at =260nm using the relation 1.0A=50ug/mL.Typically,the A260/A280 values were - 1.85.All other chemicals were of the highest available grades.
The Sunfish amphiphiles were prepared from 4-picoline and the appropriate alkyl iodides in a three-step procedure according to the methods published previously.For the current study,an array of amphiphiles(1a-1f.Table1)was chosen,differing in both tail length and unsaturation of the tails.DOPE was purchased from Avanti Polar Lipids,Inc(Alabaster,AL).Nile Red was obtained from ACROS(Landameer,The Netherlands).Asample of 7.1-kilobase DNA was isolated feom Escherichia coli using the Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands).The plasmid concentration was determined spectrophotometrically by measuring the absorption at =260nm using the relation 1.0A=50ug/mL.Typically,the A260/A280 values were - 1.85.All other chemicals were of the highest available grades.
根据前人的报道,Sunfish （太阳鱼型）两亲化合物的制备是以4-甲基吡啶与适当的烷基碘为起始原料,通过三步法得到.我们的研究所选定的一系列两亲分子（1a-1f.表1),其尾端的长度与不饱和度均不相同.DOPE由Avanti Polar Lipids,Inc(Alabaster,AL)购得,尼罗红则是从ACROS(Landameer,The Netherlands)获取.具有7100个碱基对的DNA样品是从埃希氏菌属线团分离出来的,来自Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands).质粒浓度由光谱数据测定,具体方法是测量260 nm处的吸收峰强度（按1.0A=50ug/mL换算得到）.一般地,260nm的吸收强度 / 280 nm处的吸收峰强度为1.85.其它试剂的纯度都是市面上可获得的最高纯度.
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