英语翻译Total glucose concentration was measured by phenolvitrio
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英语翻译
Total glucose concentration was measured by phenolvitriol method.One milliliter of the supernatant for each treatment was accurately taken and filled into a 10 ml cuvette.Then 0.5 ml,6% phenol was added into the cuvette and shaken-up.Then 2.5 ml vitriol was filled into the mixture in cuvette,shaken-up and stand for 30 min at room temperature.At last,absorbance values were recorded by a 722 spectrophotometer at the wavelength of 490 nm.At the same time,the wash solution was measured as blank control in an identical way.
Total glucose concentration was measured by phenolvitriol method.One milliliter of the supernatant for each treatment was accurately taken and filled into a 10 ml cuvette.Then 0.5 ml,6% phenol was added into the cuvette and shaken-up.Then 2.5 ml vitriol was filled into the mixture in cuvette,shaken-up and stand for 30 min at room temperature.At last,absorbance values were recorded by a 722 spectrophotometer at the wavelength of 490 nm.At the same time,the wash solution was measured as blank control in an identical way.
总的葡萄糖浓度用苯酚-硫酸法测量.每次治疗的上清液每一毫升都要准确提取,注入到10ml的试管中.然后,将0.5毫升6%浓度的苯酚加入试管摇匀.然后2.5毫升的硫酸倒入试管混合,摇匀并在常温下放置30分钟.最后,用波长为490转矩的722分光计测量光值.同时,测量清洗液作为空白对照.
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