大师作文网英语翻译Materials and MethodsMaterials.Rotenone,dopamine,GBR1293
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英语翻译
Materials and Methods
Materials.Rotenone,dopamine,GBR12935,N-nitro-L-arginine methyl ester hydrochloride (L-NAME),7-nitroindazole (7-NI),streptolysin O (SLO),thioflavin S,and anti-actin antibody were purchased from Sigma-Aldrich (St.Louis,MO).Anti-mouse monoclonal dopamine antibody was obtained from AbCam Limited (Cambridge,UK) and anti-mouse monoclonal nitrotyrosine antibody (clone 1A6)from Millipore (Billerica,MA).Anti-rabbit vesicular monoamine transporter2 (VMAT2) antibody was obtained from Millipore Bioscience Research Reagents (Temecula,CA).This antibody recognizes a major 80-kDa band,which represents fully processed VMAT2,and two minor bands at 62 and 40 kDa,which are also VMAT2 and presumably represent incompletely processed/glycosylated VMAT2 (Watabe and Nakaki,2007).In the present study,a major 80-kDa band of VMAT2 alone was identified.Cell Culture.SH-SY5Y cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum at 37°C
under 5% CO2 in air.
Materials and Methods
Materials.Rotenone,dopamine,GBR12935,N-nitro-L-arginine methyl ester hydrochloride (L-NAME),7-nitroindazole (7-NI),streptolysin O (SLO),thioflavin S,and anti-actin antibody were purchased from Sigma-Aldrich (St.Louis,MO).Anti-mouse monoclonal dopamine antibody was obtained from AbCam Limited (Cambridge,UK) and anti-mouse monoclonal nitrotyrosine antibody (clone 1A6)from Millipore (Billerica,MA).Anti-rabbit vesicular monoamine transporter2 (VMAT2) antibody was obtained from Millipore Bioscience Research Reagents (Temecula,CA).This antibody recognizes a major 80-kDa band,which represents fully processed VMAT2,and two minor bands at 62 and 40 kDa,which are also VMAT2 and presumably represent incompletely processed/glycosylated VMAT2 (Watabe and Nakaki,2007).In the present study,a major 80-kDa band of VMAT2 alone was identified.Cell Culture.SH-SY5Y cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum at 37°C
under 5% CO2 in air.
材料与方法
材料.鱼藤酮,多巴胺,gbr12935,精氨酸甲酯（L - NAME）,7 -硝基吲唑（7 -倪）,澳（斯洛伐克）,硫黄素,与肌动蛋白抗体购自sigma-aldrich（圣路易斯,钼）.鼠单克隆抗体是从多巴胺艾碧康有限公司（剑桥,英国）与鼠单克隆抗体（克隆中硝基）从微孔（,马）.兔囊泡单胺transporter2（VMAT 2）抗体是从微孔生物研究试剂（相似,加利福尼亚州）.这种抗体确认主要80带,这是完全处理VMAT 2,两小乐队在62和40,这也是VMAT 2和大概是完全处理/糖化VMAT 2（watabe和nakaki,2007）.在本研究中,一个主要的80带单独确定VMAT 2.细胞培养.细胞是生长在贝科的培养基辅以10%胎牛血清在37摄氏°
5%空气中二氧化碳.
材料.鱼藤酮,多巴胺,gbr12935,精氨酸甲酯（L - NAME）,7 -硝基吲唑（7 -倪）,澳（斯洛伐克）,硫黄素,与肌动蛋白抗体购自sigma-aldrich（圣路易斯,钼）.鼠单克隆抗体是从多巴胺艾碧康有限公司（剑桥,英国）与鼠单克隆抗体（克隆中硝基）从微孔（,马）.兔囊泡单胺transporter2（VMAT 2）抗体是从微孔生物研究试剂（相似,加利福尼亚州）.这种抗体确认主要80带,这是完全处理VMAT 2,两小乐队在62和40,这也是VMAT 2和大概是完全处理/糖化VMAT 2（watabe和nakaki,2007）.在本研究中,一个主要的80带单独确定VMAT 2.细胞培养.细胞是生长在贝科的培养基辅以10%胎牛血清在37摄氏°
5%空气中二氧化碳.
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