大师作文网英语翻译Before fitting a dose-response curve to obtain the EC/IC
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英语翻译
Before fitting a dose-response curve to obtain the EC/IC50,each well should be converted to either percent activity or percent inhibition with respect to positive and negative controls .Then all replicate wells from a given run (including multiple plates per run) for a given concentration should be averaged either by taking the mean,or preferably,taking the median.Outliers less influence the latter when there are 3 or more replicates.Thus only one point per concentration per run is used to fit the dose-response equation to the data.This is because replicate wells on either the same or different plates are often correlated with each other and,thus,do not provide true replication of the experiment.
Before fitting a dose-response curve to obtain the EC/IC50,each well should be converted to either percent activity or percent inhibition with respect to positive and negative controls .Then all replicate wells from a given run (including multiple plates per run) for a given concentration should be averaged either by taking the mean,or preferably,taking the median.Outliers less influence the latter when there are 3 or more replicates.Thus only one point per concentration per run is used to fit the dose-response equation to the data.This is because replicate wells on either the same or different plates are often correlated with each other and,thus,do not provide true replication of the experiment.
在拟合剂量-反应曲线以便获得EC/IC50之前,每个孔应换算为阳性对照品和阴性对照品（方面）的活性百分比或抑制百分比.然后,给定组给定浓度的所有复孔（包括每组多个板）应予以平均,要么取平均值,要么最好取中数.因为当复孔为3个或以上时,异常值对中数的影响更小一些.从而,每组每个浓度只有一个点用于拟合剂量反应方程和数据.这是由于相同或不同板上的复孔通常相互关联,而不能真正复制试验.
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